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文章摘要
林小丁,谢曦.虾青素通过线粒体抑制高糖诱导的HBZY-1细胞凋亡[J].海南大学学报编辑部:自然科学版,2019,37(1):.
虾青素通过线粒体抑制高糖诱导的HBZY-1细胞凋亡
Astaxanthin alleviates HBZY-1 cells apoptosis via mitochondria
投稿时间:2018-11-25  修订日期:2019-01-08
DOI:10.15886/j.cnki.hdxbzkb.2019.0003
中文关键词: HBZY-1细胞;虾青素;细胞活力;线粒体;细胞凋亡
英文关键词: Dalbergia odorifera T. Chen; potassium silicate; growth development; physiological characteristics
基金项目:海南省科协青年科技英才学术创新计划项目(QCXM201815)
作者单位E-mail
林小丁 海南大学热带生物资源教育部重点实验室、海南大学海洋学院药学系 linxiaoding1102@126.com 
谢曦 海南大学热带生物资源教育部重点实验室、海南大学海洋学院药学系 xiexi@hainu.edu.cn 
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中文摘要:
      为了探究虾青素是否能有效改善高糖诱导的HBZY 1细胞的凋亡及其抗凋亡的机制,本研究采用CCK8法测定了正常糖和高糖环境下,不同浓度的虾青素对HBZY 1细胞活力的影响,并运用相关试剂盒测试了细胞死活比例和细胞凋亡的变化,在光镜下观察了HBZY 1细胞形态的变化,使用JC 1探针在荧光显微镜下观察了HBZY 1细胞线粒体膜电位的变化,运用Western Blot技术检测了HBZY 1细胞中促细胞凋亡蛋白bax和抑制细胞凋亡蛋白bcl 2的表达水平,运用caspase 3/7活细胞荧光实时法检测了HBZY 1细胞中的caspase 3/7 活性.结果表明:虾青素可有效逆转高糖引起的细胞活力降低,能抑制高糖引起的HBZY 1细胞凋亡和细胞形态异常,并能改善线粒体膜电位和影响参与调控HBZY 1细胞的凋亡相关蛋白.由此认为:虾青素可以通过影响线粒体膜电位来改善高糖引起的肾小球系膜细胞凋亡,其有可能成为改善糖尿病肾病的潜在药物.
英文摘要:
      In the report, the effects of potassium silicate at different concentrations on the plant growth and physiological characteristics of Dalbergia odorifera T. Chen seedlings were analyzed. The results showed that when the concentration of K2SiO3 was 1 mmol/L, the plant growth and development of D.odorifera could be significantly improved. However, when the concentration of K2SiO3 was over 2 mmol/L, the growth and development of D.odorifera was gradually inhibited with the increment of K2SiO3 concentration. The D.odorifera seedling could change osmoregulation and increase enzyme activity to cope with certain environmental changes when plants were stressed by high concentrations of K2SiO3. The results provide the evidences that the exogenous potassium silicate can improve the growth and development of D.odorifera, and provide some important information about elucidating the effects of potassium silicate on the plant growth and development, and physiological mechanism of D.odorifera seedlings under abiotic stressed conditions.
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